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KMID : 0861020160310030043
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Korea Journal of Herbology
2016 Volume.31 No. 3 p.43 ~ p.48
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Stem bark of Maackia amurensis Extract according to extraction Solvent
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Kim Gyeong-Sun
Chang Jun-Pok
Doh Eun-Soo
Kill Ki-Jung
Yoo Ji-Hyun
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Abstract
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Objectives : The objective of this research was to investigate the antioxidant activities of stem bark of Maackia amurensis extract.
Methods : Stem bark of Maackia amurensis extract were prepared using 70% methanol. Methanol extracts were fractionated to hexane, chloroform, ethyl acetate, butyl alcohol, water fractions and investigated. The antioxidant activities of fractions was evaluated by four different assays as total polyphenol contents, total flavonoid contents, DPPH(1,1-diphenyl-2-picrylhydrazyl) free-radical scavenging activity and ABTS(2,2``-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) scavenging ability.
Results : The yield of methanolic extracts from stem bark of Maackia amurensis was 10.16%, whereas those of its solvent fractions (hexane, chloroform, ethyl acetate, butyl alcohol, and water) were 5.45, 11.39, 13.88, 26.07, and 40.80%, respectively. The total polyphenol contents and electron donating ability of 70% methanol extracts from stem bark of Maackia amurensis were 15.44 mg/g and 194.15 μg/mL of its IC50, respectively. The 70% methanol extracts showed the highest antioxidant activity. The total polyphenol content and total flavonoid content of chloroform fractions were higher in each of 201.98 mg/g and 13.55 mg/g. The chloroform fraction showed the lowest levels of DPPH(IC50, 183.95 μg/mL) and ABTS scavenging activity(IC50, 10.0 μg/mL). The antioxidant activity was detected in methanol extract, chloroform fractions.
Conclusions : These results indicate that 70% methanol extract and its fractions of stem bark of Maackia amurensis, especially chloroform fraction, have the properties of anti-oxidant suggesting stem bark of Maackia amurensis may be a candidate for natural and functional materials.
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KEYWORD
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Maackia amurensis, Antioxidant, solvent fraction
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